Draft genome of Patiria pectinifera and its genome annotation and functional annotation information

Genomic DNA was extracted from the testis of Patiria pectinifera, collected at the Misaki Marine Biological Station, using standard protocols. DNA quality and quantity were assessed using a Qubit Fluorometer and a NanoDrop spectrophotometer. Illumina paired-end libraries with an insert size of approximately 500 bp were prepared using the TruSeq DNA Sample Preparation Kit (Illumina). Mate-pair libraries with insert sizes of approximately 3 kb, 6 kb, 10 kb, and 15 kb were constructed using the Nextera Mate Pair Sample Preparation Kit (Illumina). Sequencing was performed on an Illumina HiSeq 2500 platform, generating 2 × 250 bp paired-end reads for paired-end libraries and 2 × 100 bp paired-end reads for mate-pair libraries. Genome assembly was performed using Platanus-allee v2.2.1 , followed by scaffolding with mate-pair libraries and gap closing using paired-end reads. RNA-seq was performed on brachiolaria larvae and five metamorphic stages. Sequencing reads were mapped to the Patiria pectinifera draft genome using STAR (v2.7.3a). Strand-specific transcriptome assembly was conducted using Scallop (v0.10.4), and functional annotations for the assembled transcriptome were obtained using Trinotate (v3.1.0).