The survival counts of zebrafish larvae after exposure to various concentrations of SchB from 3 to 8 dpf.

Normal WT embryos were exposed to 1.875, 3.75, 7.5 and 15 μM of SchB or DMSO carrier as controls. The treatment took place from 3 to 8 dpf. The survival of larvae in each condition was counted every day. The DMSO percentage in the controls was 0.025% and 0.2%. These were the corresponding DMSO amounts used as the drug carrier in treatment groups with 1.875 and 15 μM SchB respectively. Both DMSO percentages were lower than the commonly used percentage (60,61]. Hence, the use of these specific amounts of DMSO should not affect the survival of the larvae. Indeed, they did not. In each experimental group, 60 embryos were used. Then, the survival was counted every day from 3 to 8 dpf. The 3-dpf count was obtained immediately after the addition of chemicals.