Ilya Ravkin (2011) CIL:32083, Homo sapiens, osteocarcenoma. CIL. Dataset

This image set is of Transfluor assay where an orphan GPCR (G-protein coupled receptor) is stably integrated into a beta-arrestin GFP (green) expressing U2OS cell line also showing nucleus (red). After one hour incubation with a compound, the cells were quenched with fixative (formaldehyde) and the plate was read on Cellomics ArrayScan HCS Reader using the GPCR Bioapplication. Negative results are a more uniform distribution of GFP throughout the cytoplasm and positive results are a peri-nuclear localization.
Recommended attribution: "We used the SBS Roche Transfluor image set provided by Ilya Ravkin and available from the Broad Bioimage Benchmark Collection (www.broad.mit.edu/bbbc)."
Analysis of the data set can be downloaded from http://www.broadinstitute.org/bbbc/sbs_roche_transfluor.html

本图像集展示了Transfluor检测实验，其中孤儿G蛋白偶联受体（G蛋白耦联受体）被稳定整合至表达绿色荧光蛋白（GFP）的β-阻断素U2OS细胞系中，同时可见细胞核（红色）。在化合物孵育一小时后，细胞用固定剂（甲醛）进行淬灭处理，并使用Cellomics ArrayScan HCS Reader进行读取，应用GPCR生物应用。阴性结果表现为细胞质中GFP分布更为均匀，而阳性结果则显示GFP在细胞核周围的定位。推荐引用信息：'本实验使用了Ilya Ravkin提供的SBS Roche Transfluor图像集，该图像集可通过Broad Bioimage Benchmark Collection（www.broad.mit.edu/bbbc）获取。数据集分析可从http://www.broadinstitute.org/bbbc/sbs_roche_transfluor.html下载。'