EFM detection of lipid bodies (LBs) in C57BL/6 DLs.
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https://figshare.com/articles/dataset/_EFM_detection_of_lipid_bodies_LBs_in_C57BL_6_DLs_/719525
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Controls (left panels) and L. am amastigote-loaded DLs (right panels) were incubated or not with oleate as described previously. Cells were then detached, deposited on coverslips, and LBs were evidenced by the incorporation of BODIPY 493/503 2 µg/ml in PBS for 30 minutes at 34°C (in green). Cells were fixed and stained with M5/114 mAb (in red). DL and amastigote nuclei were stained with Hoechst 33,342 (blue spots indicated by arrowheads).
创建时间:
2016-02-24



