Metabarcoding to Detect Invasive Fishes data

Data associated with a DNA metabarcoding study of fish (invasive and native) biodiversity in four rivers in Ontario

Sub directories

00_sample_map: Map of sampling locations and abundance of larval fishes collected at each site

01_database_build: Reference genetic database and taxonomic information for classification of metabarcoding sequencing reads

02_qiime2_analysis: De-noising and dereplicating DNA metabarcoding data

03_read_correction: Correction for contamination using no-template controls and single fish samples

04_sampling_stats: General summary statistics of sequencing

05_river_richness: Summaries and analyses of species richness across rivers

06_gear_richness: Comparison of gear types on species detected

07_primer_consistency: Comparison of primer target gene on species detected

08_sampling_depth: Comparison of number of samples collected to read depth per sample

09_database_comparison: Comparison of the reference databases for COI and 12S

10_amplicon_distance: Comparison of the genetic distance for COI and 12S metabarcoding primers