Transcriptome-wide profiling of N6-methyladenosine during shade avoidance response in Arabidopsis

Shade avoidance is a critical adaptive strategy in plants, enabling rapid growth response to access more sunlight under crowding. Despite N6-methyladenosine (m6A) is the most abundant internal modification of eukaryotic mRNA and plays critical roles in RNA stability and translation, m6A dynamics and role in shade avoidance remains unclear. To investigate the effect of shade on m6A modification, we performed methylated RNA immunoprecipitation sequencing (MeRIP-seq) on wild-type Arabidopsis seedlings grown under continuous white light and subjected to 8 h of shade treatment, as well as on the phyB-9 mutant under white light. The results revealed that global m6A modification levels were significantly reduced either after shade treatment or in the phyB-9 mutant. Overall design: 7-day-old Col-0 seedlings exposed to shade for 8 h and phyB-9 mutant grown under continuous white light