Supplemental Tables S1-S5

Regular aerobic exercise decreases the risk of metabolic diseases, however reduced physical activity may attenuate these beneficial adaptations. This study investigated the influence of a single day of reduced activity on the skeletal muscle transcriptome (RNASeq) and systemic metabolism surrounding an acute bout of aerobic exercise. Utilizing a crossover design, subjects (n=9 [7M, 2F], 24±1y) exercised at 65% VO2max for 60-minutes following a day of reduced activity (IN; 3,581±1,185 steps) and normal activity (A; 11,069±3,631 steps). RNAseq from skeletal muscle biopsies was completed prior to and 4-hours post-exercise. Respiratory gas exchange analysis was completed at 20, 40, and 60 minutes of exercise. Blood samples for select metabolites were collected before, during, and throughout the 4-hour recovery. Inactivity resulted in nine Differentially Expressed Genes (DEG) at rest, while exercise altered 793 and 1,403 genes in A and IN, respectively. Gene set enrichment analysis revealed an interaction of exercise and trial that were related to inflammation, metabolism, cell cycle, and innate immunity. Respiratory exchange ratio (0.93±0.04 vs. 0.91±0.03; p