O-GlcNAcylation governs astrocytes reactivation and involves in Alzheimer's disease

To reveal the mechanisms that Ogt controls inflammatory response of astrocytes, we first performed RNA-seq with Ctrl and Ogt cKO astrocytes isolated from the brains of adult mice. Given the inflammation in the brain of Alzheimer's disease, we next performed RNA-seq with astrocytes isolated from the brains of adult Ctrl and 5X APP Alzheimer's mice model (AD), and Aß treated astrocytes, respectively. Taken together, these findings suggest that differentially expressed genes shared between cKO astrocytes, AD astrocytes and Aß-treated astrocytes are related with inflammation. Overall design: Two replicates of mRNA in astrocytes isolated from the brains of adult Ctrl and Ogt cKO mice, 5-month-old Ctrl and 5X APP Alzheimer's mice model (AD), Ctrl and Aß treated adult astrocytes, respectively