To explore the co-pathogenesis of obesity and nonalcoholic steatohepatitis based on bioinformatics analysis

Objective Bioinformatics methods were used to investigate the pathogenesis,disease-characteristic genes and immunoinvasive manifestations of obesity(OB)and nonalcoholic steatohepatitis(NASH), and to explore the correlation between disease-characteristic genes and immune cells.Methods OB and NASH related chips were obtained from GEO database,R language was used to analyze gene differences and WGCNA analysis,GO and KEGG enrichment were analyzed by intersection analysis,and protein-protein interaction network was constructed at the same time.Key genes were selected using 12 cytohubba methods,ROC curve and sample chip were used to detect the accuracy of key genes,and the disease characteristic genes with the best performance were selected.CIBERSORT algorithm was continued to analyze the immune infiltration of OB and NASH,and the correlation between disease characteristic genes and immune cells was analyzed.Results A total of 235 differential genes were obtained in the obesity training group GSE25401 and GSE151839,and 804 differential genes were obtained in the non-alcoholic steatohepatitis training group GSE63067 and GSE89632.GO analysis mainly involved the significant expression of interleukin 8 regulation.KEGG analysis showed that multiple comb inhibition complex and other pathways were closely related to OB and NASH.Key genes IL6,IL1B,IL1RN,VCAN and TNFAIP6 were selected by 12 cytohubba methods.ROC curve and sample chip were used to detect disease characteristic genes,and VCAN and IL1RN had the best effect.Conclusion: OB and NASH characteristic genes VCAN and IL1RN are significantly correlated with immune cells,which provides a preliminary basis for further research on OB and MASH targeted diagnosis and treatment.