ETMR stem-like state and chemoresistance are supported by perivascular cells at single-cell resolution

To investigate the cellular heterogeneity of ETMR and the role of pericytes in the tumor microenvironment, we performed sequencing at single-cell resolution of about 250,000 cells from primary human and murine ETMR as well as from 2D ETMR cultures and 3D models of ETMR-harboring forebrain organoids. We also analysed 3 ETMR human samples by spatial transcriptomics. Gene expression profiles of human ETMR (n=9) were generated of formalin-fixed, paraffin-embedded (FFPE) tissue samples using Chromium Fixed RNA Profiling for Multiplexed Samples technology (10X Genomics).