Next Generation Sequencing Quantitative Analysis of altered expression of genes in KRASG12D PDAC tissues

KRAS mutation is the most frequently mutated oncogene, occurring in approximately 90% of patients with PDAC, where KRASG12D is the most common allele in PDAC. Recent studies revealed that KRASG12D mutation caused the aberrant gene expression profile by regulating RNA processing to drive tumor progression (9, 17). However, the role of the KRASG12D mutation in triggering the circRNA process during tumor development remains unknown. Our study aims to explore the genes that regulates circRNA biogenesis in the LN metastasis of PDAC. To detect the gees that regulates circRNA biogenesis in LN metastasis of PDAC, the total RNA of KRASG12D PDAC tissues and paired normal adjacent tissues was extracted and the next generation sequencing was conducted to explore the expressing profile difference between PDAC tissues and paired normal adjacent tissues. The result was obtained from three independent experiments.