Glycan-based Biological Degraders Targeting the Cytokine Immune Axis

The project investigates targeted degradation of IL-6 and sIL6-R using bispecific Biological Degraders (BioDegs) that enable receptor-mediated uptake via the asialoglycoprotein receptor (ASGPR). Various IL-6(R)–binding scaffolds were modified with triantennary N-acetylgalactosamine (TGN) to promote lysosomal targeting. Mass spectrometry was used to determine the glycan-to-protein ratio after modification of the different binders, providing analytical characterization of the conjugates and confirming successful generation of BioDeg constructs.