Melatonin supplementation protects against traumatic colon injury through regulating Serpina3n protein expression

DescriptionMetagenome: This file displays metagenomic sequencing data of intestinal microbiota in the sham and TCI groups. Proteomics: This file displays proteomics data of the intestinal epithelium in the sham and TCI groups. Figure 1: This file displays survival time of the sham and TCI groups within 96 hours. The relative abundances of Shigella, Escherichia and Salmonella between the sham and TCI groups were also exhibited. Figure 2: This file displays the IHC score of Spera3n expression in the intestinal epithelium of the sham and TCI groups. Figure 3: This file displays the following data: (1) This file displays survival time of the TCI, TCI+KO and Sham+KO groups within 96 hours (Figure 3B). (2) The concentrations of IL-6 and TNF-α in plasma and intestines of WT or Serpina3n KO mice that received sham or TCI operation (Figure 3C,3D). (3) The IHC score of both ZO-1 and occludin expression in the intestinal epithelium of mice as in (2) (Figure 3E). (4) The relative abundances of Shigella, Escherichia and Salmonella of mice as in (2) (Figure 3F). Figure 4: This file displays the following data: (1) This file displays survival time of the TCI, Sham+MLT and TCI+MLT groups within 96 hours (Figure 4B). (2) Concentrations of IL-6 and TNF-α in the plasma and intestines of Vehicle- or MLT-fed mice undergoing sham or TCI surgery. (Figure4C,4D). (3) The IHC score of both ZO-1 and occludin expression in the intestinal epithelium of mice as in (2) (Figure 4E). (4) The relative abundances of Shigella, Escherichia and Salmonella of mice as in (2) (Figure 4F). Figure 5: This file displays the following data: (1) The relative cell migration rate of Caco-2 cells treated with vehicle, MLT, JSH-23, JSH-23+MLT (Figure 5E). (2) The percent survival of mice in the TCI groups that were administered with JSH-23 or MLT (Figure 5F). (3) The IHC score of Spera3n expression in the intestinal epithelium as in (2) (Figure 5H). Figure S1: The relative abundances of Cronobacter and Proteus between the sham and TCI groups were also exhibited. Figure S2: The relative cell migration rate of vector, Serpina3n, shNC and shSerpina3n groups. Figure S3: The IHC score of Spera3n expression in the intestinal epithelium of WT and Serpina3n KO groups. Figure S4: The percent survival of Serpina3n KO mice that received sham or TCI operation and WT mice that received TCI operation.Figure S5: The concentrations of CRP in plasma of WT or Serpina3n KO mice that received sham or TCI operation. Figure S6: The concentrations of DAO, D-lactate and Endotoxin of WT or Serpina3n KO mice that received sham or TCI operation. Figure S7: The relative abundances of Cronobacter and Proteus of Sham+WT, Sham+KO, TCI+WT and TCI+KO. Figure S8: This file displays the following data: (1) The relative cell migration rate of Caco-2 cells treated with vehicle 0.01 μM, 0.1 μM or 1 μM MLT (Figure S8B). (2) The IHC score of Spera3n expression in the intestinal epithelium of of the TCI, Sham+MLT and TCI+MLT groups (Figure S8C). Figure S9: (1) The relative cell migration rate of Vector, MLT, Serpina3n and Serpina3n+MLT (Figure S9C). (2) The percent survival of mice in the sham or TCI groups that were administered with vehicle or MLT (Figure S9D). Figure S10: Concentrations of CRP in the plasma and intestines of Vehicle- or MLT-fed mice undergoing sham or TCI surgery. Figure S11: The concentrations of DAO, D-lactate and Endotoxin of Vehicle- or MLT-fed mice undergoing sham or TCI surgery. Figure S12: The relative abundances of Cronobacter and Proteus of Sham+Vehicle, Sham+MLT, TCI+Vihicle and TCI+MLT.