Single Cell RNA Sequencing of MIC WT and B1KO early invasive breast carcinoma. Single Cell RNA Sequencing of MIC WT and B1KO early invasive breast carcinoma

The goal of this study is the compare the tumour compositions between B1 integrin-proficient and -deficient dormant lesions based on single cell transcripts. Tumours were dissociated and pooled into single cell suspension, passed multi-stage quality checks, and sequenced by Génome Québec Innovation Tumour. Clustering visualization by UMAP and differential gene expression analyses show that B1 integrin-deficient dormant lesions have significantly more cancer-associated fibroblasts by depleted of antitumourigenic immune cells, altogether promoting dormnacy exit and recurrence. Overall design: Sequencing on viable single cell suspension from pooled mouse mammary early invasive lesions (B1 integrin-proficient or -deficient)