Dysregulation of cell state dynamics during early stages of serous endometrial carcinogenesis

We have compiled single-cell transcriptomes of the mouse endometrium
before and after induction of serous endometrial carcinoma (SEC) using our
genetically engineered mouse model. In this model, Pax8 is the
promotor for the targeted inactivation
of Trp53 and Rb1 in the mouse endometrium after
the introduction of doxycyline to recapitulate SEC. To prepare a census of
single-cell RNA and spatial RNA expression profiles, we collected uterine
horns from 18 mice (6 weeks to 6 months old) including 5 normal, 7
pre-dysplastic (30-50 days post-induction, DPI), and 6 dysplastic (80-150
DPI) samples. For spatial transcriptome evaluation we collected uterine
horns from 13 mice including 4 normal, 4 pre-dysplastic, and 5 dysplastic
samples. All mice were at late diestrus according to vaginal smears
followed by morphological verification and estimation of Ki67 index in
paraffin sections of adjacent uterus. Additionally, pathology of each
sample was validated by the analysis of trained comparative pathologists
to identify if they are show dysplastic lesions. The datasets included in
this upload are seurat objects of all samples combined, as well as those
separated by SEC stage (Normal, Pre-dysplastic, or Dysplastic). Each stage
has an object associated with all cells and an epithelial subset.
Additionally, there are two RDS objects included that are the combined
sample dataset at two stages throughout the quality control process. The
Visium datasets included are .cloupe files compatable for evaluation in
LoupeBrowser 7.