Identification of biotinylated SGC surface proteinsa).

a) MS data were first screened against the Acropora digitifera protein library (see the section of “Materials and methods”). Matched coral proteins with MS>23 were then blasted to NCBInr database to identify predicted proteins.b) The serial no. of proteins in Acroporal digifera database (http://marinegenomics.oist.jp/genomes/downloads?project_id=3).c) Transmembrane domains were predicted by TMpred. (http://www.ch.embnet.org/software/TMPRED_form.html).d) MOWSE score/number of total matched peptides (numbers of different matched peptides) against the Acropora digitifera protein library.e) The coverage of protein spot peptide sequence among matched Acropora protein.f) The percentage of sequence identity between the identified Acropora protein and the closet matched protein in NCBInr database.g) The species were protein spot to blast NCBInr database using the MASCOT search program, and this column shown the closest matched species.h) Relative fluorescent ratio (fold) of the Alexa Fluor 488 (i.e. biotinylated) over SYPRO (see the “Materials and methods” section).