Raw Data Summary of AGPAT1 article

This study aims to elucidate the oncogenic role and molecular mechanism of the phospholipid metabolism enzyme AGPAT1 in head and neck squamous cell carcinoma (HNSCC). This dataset contains a summary of all raw experimental data supporting the conclusions of the associated manuscript, including:Clinical Sample Information: Correlation data between clinicopathological characteristics (gender, age, stage, grade, etc.) of 92 HNSCC patients and AGPAT1 mRNA expression (qPCR, n=70) and protein expression (IHC, n=80).Cell Functional Assay Data: Raw measurements of the effects of AGPAT1 knockdown and overexpression on the proliferation (CCK-8) and apoptosis (flow cytometry) of HNSCC cells (e.g., TU686).Molecular Mechanism Experiment Data:Western Blot: Grayscale values for protein expression of AGPAT1, PA/PC metabolites, key Hippo pathway proteins (LATS1, YAP1) and their phosphorylated forms.Co-IP Assay: Raw binding intensity data verifying the effect of PC treatment on the interaction between YAP1 and nuclear transport proteins Importin α/β.qPCR: Relative mRNA expression levels of YAP1 downstream target genes (CTGF, BIRC5, CYR61).Animal Experiment Data: Dynamic measurements of tumor volume and weight, as well as intratumoral molecular marker data from a subcutaneous xenograft mouse model with three groups: sh-AGPAT1, sh-AGPAT1 + PA/PC, and sh-AGPAT1 + OE-YAP1.The data is organized in structured tables (e.g., Excel) for easy re-analysis and verification. This dataset provides comprehensive raw data supporting the hypothesis of the “AGPAT1-PA/PC-YAP1 signaling axis driving HNSCC progression.”