Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in the Drosophila mushroom body contributing to olfactory learning

O-GlcNAcylation is a dynamic post-translational modification that diversifies the proteome. Its dysregulation is associated with neurological disorders that impair cognitive function, and yet identification of phenotype-relevant candidate substrates in a brain-region-specific manner remains unfeasible. By combining an O-GlcNAc binding activity derived from Clostridium perfringens OGA (CpOGA) with TurboID proximity labeling in Drosophila, we developed an O-GlcNAcylation profiling tool that translates O-GlcNAc modification into biotin conjugation for tissue-specific candidate substrates enrichment. We mapped the O-GlcNAc interactome in major brain regions of Drosophila and found that components of the translational machinery, particularly ribosomal subunits, were abundantly O-GlcNAcylated in the mushroom body of Drosophila brain. Hypo-O-GlcNAcylation induced by ectopic expression of active CpOGA in the mushroom body decreased local translational activity, leading to ol..., , , # Data from: Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in the *Drosophila* mushroom body contributing to olfactory learning

DOI:[10.5061/dryad.sj3tx969t ](https://doi.org/10.5061/dryad.sj3tx969t)

This dataset includes the data in the above publication. In this publication, we developed an *O*-GlcNAcylation profiling tool that translates *O*-GlcNAc modification into biotin conjugation for tissue-specific candidate substrate enrichment. We mapped the *O*-GlcNAc interactome in major brain regions of *Drosophila* and found that components of the translational machinery, particularly ribosomal subunits, were abundantly *O*-GlcNAcylated in the mushroom body of ...,