Gene expression of ex vivo cultured osteoclasts during the course of differentiation

The aim of this analysis was to investigate the changes in the gene expression pattern of ex vivo cultured wildtype murine osteoclasts during the course of osteoclastogenic differentiation. Primary osteoclasts were obtained from 12 week old female C57Bl/6J mice. Bone marrow was extracted and cultured in a-MEM including 10 % FBS and 10 nM vitmain-D3 for 3 days. Thereafter they were cultured in a-MEM including 10% FBS, 10 nM Vitamin-D3, 20 ng/ml MCSF and 40 ng/ml RANKL. The expression patterns of these cells was compared at four timepoints during the course of differentiation (day 1, day 4, day 5 and day 7).