Innate triggering and antiviral effector functions of activin A

5A:2^dCt GAPDH-INHBA expression following transfection of human PBMC (peripheral blood mononuclear cells) with IVT-RNA or poly(I:C) at two doses5B:2^dCt GAPDH-MX1 expression following transfection of human PBMC with IVT-RNA or poly(I:C) at two doses5C:2^dCt GAPDH-IFI6 expression following transfection of human PBMC with IVT-RNA or poly(I:C) at two doses5D:2^dCt GAPDH-INHBA expression following exposure of human PBMC to the imidazoquinolones R848 and R8375E:2^dCt GAPDH-MX1 expression following exposure of human PBMC to the imidazoquinolones R848 and R8375F:2^dCt GAPDH-INHBA expression following transfection of human PBMC with ssRNA40 LyoVec (TLR8 agonist)5G:2^dCt GAPDH-IFI6 expression following transfection of human PBMC with ssRNA40 LyoVec (TLR8 agonist)6A:2^dCt GAPDH-INHBA at 48 hours post-infection in A549.gfp cells infected with increasing titres of Sendai virus (SeV) compared to controls6B:2^dCt GAPDH-MX1 at 48 hours post-infection in A549.gfp cells infected with increasing titres of Sendai virus (SeV) compared to controls6C:2^dCt GAPDH-INHBA expression in HuH7 (RIG-I replete) versus HuH7.5 (RIG-I deficient) cells following SeV infection at various multiplicities, assayed at 48 hours post-infection.6D:2^dCt GAPDH-MX1 expression in HuH7 (RIG-I replete) versus HuH7.5 (RIG-I deficient) cells following SeV infection at various multiplicities, assayed at 48 hours post-infection.6E:2^dCt GAPDH-SEV (encoding Sendai virus genomic RNA) expression in HuH7 and HuH7.5 cell lines, assayed at 48 hours post-infection6F:Fold change in INHBA expression, normalized to GAPDH, at 72 hours post-infection in HuH7.5.1 cells relative to uninfected (U.I.) cells.Figure 6G:Fold change in MX1 expression, normalized to GAPDH, at 72 hours post-infection in HuH7.5.1 cells relative to uninfected (U.I.) cells.7A:2^dCt Hprt-Inhba expression in the lungs of FLUAV PR/8 infected mice at 72 hours post-infection.p { margin-bottom: 0.25cm; direction: ltr; color: #000000; line-height: 115%; text-align: left; orphans: 2; widows: 2; background: transparent }p.western { font-family: "Liberation Serif", serif; font-size: 12pt; so-language: en-GB }p.cjk { font-family: "Noto Serif CJK SC"; font-size: 12pt; so-language: zh-CN }p.ctl { font-family: "FreeSans"; font-size: 12pt; so-language: hi-IN }7B:2^dCt Hprt-Isg15 expression in the lungs of FLUAV PR/8 infected mice at 72 hours post-infection.7C:Correlation of Inhba and Isg15 transcript induction in the lungs of FLUAV PR/8 infected mice at 72 hours post-infection (linear regression, R2 = 0.9271, p=0.002)7D:2^dCt Hprt-Inhba expression in the liver of FLUAV PR/8 infected mice at 72 hours post-infection7E:2^dCt Hprt-Isg15 expression in the liver of FLUAV PR/8 infected mice at 72 hours post-infection7F:2^dCt Hprt-Inhba induction in the lung tissue of FLUAV PR/8 infected mice at 48 hours post-infection, across a range of increasing multiplicities of infection. 7G:2^dCt Hprt-Inhba expression in wildtype or MAVS-depleted mice at 48 hours post-infection with FLUAV PR/87H:2^dCt Hprt-Isg15 expression in wildtype of MAVS-depleted mice at 48 hours post-infection with FLUAV PR/8Supplementary Figure 1A:Fold change INHBA normalized to GAPDH for HuH7 cells exposed to recombinant IFN or control medium (D10)Supplementary Figure 1B/C/D: OR6 cells incubated with a titration of IFNα in the presence of titrating doses of activin A; assayed for Renilla luciferse and Cell Titer Glo activity.