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Diet characterization of the Mexican harbor seal using high-throughput DNA sequencing

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NIAID Data Ecosystem2026-03-10 收录
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We characterized the diet of the Punta Banda Estuary harbor seal colony (Mexico:Baja California) through metabarcoding. Briefly, we used a two-step PCR method, included the V9 region in eukaryotes and 16S mitochondrial region of Chordata and cephalopods species. A blocking oligonucleotide matching harbor seal sequences was used in the first PCR to prevent seal DNA amplification.

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2019-01-31
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