A global transcriptome analysis of human epidermal keratinocytes upon overexpression of microRNA-34a or microRNA-34c

miR-34a and miR-34c were found up-regulated at wound-edges of human venous ulcer compared to nomal wound and the intact skin; however their biological role in keratinocytes during wound repair has not been studied. To study the genes regulated by miR-34a and miR-34c, we transfected miR-34a and miR-34c mimic into human primary epidermal keratinocytes to overexpress them. We performed a global transcriptome analysis of keratinocytes upon overexpression of miR-34a or miR-34c using Affymetrix arrays. Expression profiling of primary human epidermal keratinocytes transfected with 20nM miR-34a mimics (miR-34a) or 20nM miR-34c mimics (miR-34c) or 20nM control oligos (miR-con) for 48 hours was performed using Affymetrix GeneTitan system.