Phenotypic data from seminal fluid protein Acp29AB knockdown experiments

Seminal fluid proteins (SFPs) can alter female physiology and behavior, with consequences that may align with or oppose female interests. Many SFPs are known to shape aspects of the female post-mating response, yet evidence for direct effects on female fitness is scarce. In Drosophila melanogaster, the SFP Acp29AB is firmly linked to sperm defense (P1) and sperm storage, but its impacts on females are poorly understood. Using a GAL4–UAS RNAi system with perfectly matched genetic backgrounds, we manipulated Acp29AB expression either ubiquitously or specifically in the male accessory gland and then asked how knockdown alters male sperm competition and, in female mates, oviposition dynamics and competitive fitness. Both knockdowns impaired male P1, consistent with past work on Acp29AB. We uncovered a previously undetected effect on oviposition timing: across 14 days after a single mating, females mated to control males laid more eggs early in life than females mated to knockdown males, adv..., , # Phenotypic data from seminal fluid protein Acp29AB knockdown experiments

Dataset DOI: [10.5061/dryad.c2fqz61pz](https://doi.org/10.5061/dryad.c2fqz61pz)

## Description of the data and file structure

Expression of the seminal fluid protein gene Acp29AB was knocked down in Drosophila melanogaster using RNAi driven by two different GAL4 drivers, one ubiquitous and one tissue-specific. Knockdown was validated by qPCR, and male and female phenotypes, including sperm competition (P1 and P2), egg-laying across 14 days, and female competitive fitness, were measured.

### Files and variables

#### File: Acp29AB_eggs_ovu.txt

**Description:** 14-day egg-laying time course for females mated to males with reduced Acp29AB expression (ovu driver).

##### Variables

* vial: individual female
* treatment: control (GFP) versus knockdown (29AB) mate
* day: day of egg-laying, 1-14
* eggs: number of eggs counted

#### File: Acp29AB_eggs_ubi.txt

**Description:** 14-day egg-laying time course for femal...,