Transcriptome study of MX1 overexpression on down syndrome derived aminocytes(DSACs)

MX1 mediated AP-1 involved transcriptional regulation play an important role in the pathogenesis of DS through integration analysis of ATAC-seq and RNA-seq. To confirm the function of MX1 in DS, we applied RNA-seq to explore the difference between MX1-OE and Blank group Overall design: Human MX1 coding sequence (CDS, Gene ID:4599) was cloned into the lentivirus backbone pcSLenti-EF1-EGFP-F2A-Puro-CMV-MCS-WPRE to construct MX1-OE plasmid (OBIO Biosciences, Shanghai, China). MX1-OE lentivirus was produced through MX1-OE plasmid transfection into HEK-293 T cells together with the packaging plasmids. Then the Normal aminocytes were infected with MX1-OE lentivirus and backbone lentivirus infected aminocytes were used as Blank group.