MacroH2A1.2 inhibits prostate cancer-induced osteoclastogensis through cooperation with HP1a and H1.2

Prostate cancer cells frequently metastasize to bone and secrete signaling factors that can lead to osteoclast differentiation. However, the mechanism of regulation of ostecolastostogenesis by soluble secretory factors from prostate cancer is largely unknown. MacroH2A effect the chromatin function and can potentially attenuate the prostate cancer induced ostoclastogenesis. To study the effect of mH2A1.2 on osteclastogenesis , we used genome wide gene expression studies in wild type and mH2A1.2 knockout DU154 – prostate cancer cells. Total RNA was isolated from DU154 cells in which mH2A1.2 gene have been depleted along with the wild type cells to study the differenetial regulation of various genes in each case.