Metadata record for the article: Genetic interactions among Brca1, Brca2, Palb2 and Trp53 in mammary tumor development

<b>Summary</b><br>

This
metadata record provides details of the data supporting the claims of the
related article: “Genetic interactions among Brca1,
Brca2, Palb2 and Trp53 in mammary tumor development”.

The
related study conducted parallel conditional knockout (CKO) of Brca1, Palb2 and
Brca2, individually and in combination, along with one copy of Trp53, in the
mammary gland of nulliparous female mice in order to directly compare the
latency and penetrance of tumour development associated with each gene and the
histopathological and genomic features of the mutant tumours in the same
setting.

Type of data:
whole exome sequencing, analyses of mouse mammary glands and cultured human
cells; flow cytometry

Subject of
data: mouse of mixed background; DAOY cells from ATCC

Sample size:
Number of mice used (n=~25 per genotype) was determined empirically. Number of tumours sequenced (n=5 per host
genotype) was determined empirically and by available financial resources.

<b>Data
access</b>

The whole
exome sequencing data are openly available in the <i>Sequence Read Archive</i> via the following accession: https://identifiers.org/ncbi/insdc.sra:SRP199480
(BioProject accession: PRJNA544737).

Supplementary Table S1, which underlies
Figure 1 and Table 1 of the related article, is shared openly with this data
record in the Excel file ‘Table S1.xlsx’.

Original blots underlying Figure 5 are
also shared openly with this data record in the PDF files ‘Figure S4.pdf’ and ‘Figure
S5.pdf’.

<b>Corresponding author(s) for this study</b>

Bing
Xia, Rutgers Cancer Institute of New Jersey, 195 Little Albany Street, New
Brunswick, NJ 08903. Phone: [phone]; Email: [e-mail]

Britta
Weigelt, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY
10065. Phone: [phone]; Email: [e-mail]

<br>
<b>Ethics oversight </b>

IACUC
of Rutgers Robert Wood Johnson Medical School