scRNAseq analysis in control and Goblet-Y/T-Scgb1a1CreERT mouse lung tissues.

This experiment aims to examine the global cellular response in the lung tissues of Goblet-Y/T-Scgb1a1CreERT mice. Depletion of Yap and Taz in airway secretory cells resulted in goblet cell metaplasia. To elucidate the underlying cellular response, we utilized scRNA sequencing of lung tissues. Scgb1a1CreERT+ and Scgb1a1CreERT- mice were administered daily intraperitoneal tamoxifen injections for five consecutive days. Seven days after the final dose, the lungs were harvested and processed into single-cell suspensions. The cells were then stained with fluorescently conjugated CD45 antibodies, and CD45+ and CD45- cells were isolated and mixed in a 1:1 ratio for scRNA sequencing.