Triple-combination therapy for cutaneous leishmaniasis using detergent-free, hyaluronate-coated elastic nanovesicles - Supplementary data set

Aim: To develop and evaluate detergent-free, triple-drug-loaded, hyaluronate-coated elastic nanovesicles
(H-ENVs) for the topical treatment of cutaneous leishmaniasis. Materials & methods: H-ENVs were
developed and evaluated for vesicle size, entrapment efficiency, skin permeation and antileishmanial
potential. Results: A 15.7 and 28.6% decrease in the cytotoxicity of paromomycin and amphotericin B,
respectively, was observed in detergent-free ENVs compared with conventional ENVs. H-ENVs improved
the efficacy of paromomycin against promastigote and amastigote models of leishmaniasis by 4- and
7.5-fold, respectively. In vivo investigation of H-ENVs demonstrated efficient topical management of
cutaneous leishmaniasis. Conclusion: The results indicate the potential of H-ENVs as a safe topical
treatment choice for cutaneous leishmaniasis.