Dissection of a CTCF insulator and its impact on chromosome topology and oncogene expression in gastrointestinal stromal tumor (micro-C)

Enhancer-Promoter communication is dependent on the topological organization of genomic DNA, including topologically-associated domains (TADs) and boundaries enforced by the CTCF insulator protein. Here, we characterized the structure and function of the FGF3/4 locus and the boundary element that insulates the region from an enhancer in the adjacent TAD. Overall design: To investigate the topological impact of CTCF insulator disruptions, we established derivatives of the GIST-T1 cell line. We then performed region-capture micro-C assay in GIST-T1 WT and insulator KO cells.