C1 single-cell RNA-seq of immature (P6) ID4-EGFP mouse spermatoognia

To reveal distinct transcriptomes associated with spermatogonial stem cell renewal vs. initiation of differentiation, single-cell transcriptomes from P6 ID4-EGFP+ spermatogonia were subdivided into subpopulations that displayed distinct fates when assayed by transplantation, with ID4-EGFPbright cells highly enriched for SSCs, and ID4-EGFPdim cells enriched for progenitors. We used the Fluidigm C1 instrument to capture individual spermatogonia for SMART-Seq2 single-cell RNA-seq.为揭示与精原干细胞（spermatogonial stem cell, SSC）自我更新及分化启动相关的特异性转录组特征，本研究针对出生后第6天（P6）的ID4-EGFP阳性精原细胞的单细胞转录组进行亚群细分，所得到的亚群经移植实验验证后呈现出不同的分化命运；其中ID4-EGFP亮阳性细胞显著富集SSCs，而ID4-EGFP弱阳性细胞则富集精原祖细胞。实验采用Fluidigm C1单细胞捕获系统，通过SMART-Seq2技术完成单个精原细胞的单细胞RNA测序。