Aly/REF interacting lncRNAs and mRNAs by RNA immunoprecipitation (RIP)-sequencing in human abdominal aortic aneurysm tissue

We performed a global screening for Aly/REF-binding lncRNAs and mRNAs in an infrarenal abdominal aortic aneurysm patient (maximum aortic diameter=64.3 mm) sample, who have no comorbidity i.e. cancer, drug history, infection, or any other immune-related disease that might have influenced the study. After screening for reads in AAA tissue compare with anti-Aly/REF group relative to IgG as control. The clustered libraries were loaded onto a reagent cartridge and forwarded for sequencing runs on an illumina Hiseq 4000. Human infrarenal abdominal aortic aneurysm sample lncRNA and mRNA profiles of binding interactions with Aly/REF by RNA immunoprecipitation sequencing.