Calcium imaging in visual cortex during fidget behaviors in mice

Multiple recent studies have shown that motor activity greatly impacts the activity of primary sensory areas like V1. Yet, the role of this motor related activity in sensory processing is still unclear. 
Here we dissect how these behavior signals are broadcast to different layers and areas of the visual cortex. To do so, we leveraged a standardized and spontaneous behavioral fidget event in passively viewing mice. Importantly, this behavior event had no relevance to any ongoing task allowing us to compare its neuronal correlates with visually relevant behaviors (e.g running). 
A large two-photon Ca2+ imaging database of neuronal responses uncovered four neural response types during fidgets that were consistent in their proportion and response patterns across all visual areas and layers of the visual cortex. Indeed, the layer and area identity could not be decoded above chance level based only on neuronal recordings. In contrast to running behavior, fidget evoked neural responses that we..., This dataset is a collection of fidget aligned neural events collected from large scale calcium imaging in the mouse brain.
Transgenic mice

All animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) at the Allen Institute for Brain Science. Triple transgenic mice (Ai93, tTA, Cre) were generated by first crossing Ai93 mice with Camk2a-tTA mice, which preferentially express tTA in forebrain excitatory neurons. Double transgenic mice were then crossed with a Cre driver line to generate mice in which GCaMP6f expression is induced in the specific populations of neurons that express both Cre and tTA.
Rorb-IRES2-Cre;Cam2a-tTA;Ai93 (n=10) exhibit GCaMP6f in excitatory neurons in cortical layer 4 (dense patches) and layers 5,6 (sparse). Cux2-CreERT2;Camk2a-tTA;Ai93 (n=16) expression is regulated by the tamoxifen-inducible Cux2 promoter, induction of which results in Cre-mediated expression of GCaMP6f predominantly in superficial cortical layers 2, 3 and 4. Sl..., Matlab and/or Python are needed to open data. This data submission includes 144 mat files. They can be accessed with the matlab or python function \"load\". Files are named Fidget_Fluorescence_XXXXXXXX.mat where XXXXXXXX represents the session number.Â