Amplification of DNA Brownian Motion Using Electrokinetic Noise

This dataset was collected to study the effects of applied white Gaussian electrokinetic noise on the diffusivity of single DNA molecules inside a nanofluidic slit. Our raw data consists of image sequences in FITS format (Flexible Image Transport System). Each image shows a fluorescent Lambda DNA molecule stained by YOYO-1 dye at a 10:1 base-pair to dye ratio diffusing inside a 110 nm deep nanoslit under specified amounts of applied noise. An imaging system comprising an inverted Nikon TE2000U microscope, an ANDOR iXon 897 EMCCD camera, an X-Cite UV illumination system, and a 60X, 0.7 NA, water-immersion objective lens with an extra 1.5X magnification factor was used to acquire the images. We applied 8 different noise intensities and recorded about 50-70 molecules per noise intensity. These data can be used to find trajectories of the centers of mass of the molecules and to analyze the radii of gyration and DNA shapes. Sigma represents the standard deviation of the applied Gaussian noise.