HT-SELEX and genomic HT-SELEX rawdata for experiments performed with 4 C2H2 zinc finger transcription factors for the study: " Alternate binding modes of human C2H2 TFs"

Study describes (Abhi and al., please add something here about the paper). High throughput Systematic Evolution of Ligands Through Exponential Enrichment (HT-SELEX) method was modified from approaches described in Jolma et al.(2010, 2013 and 2015) and Nitta et al. 2015. (PMID: 20378718, PMID: 23332764, PMID: 26550823 and PMID: 25779349). In this study we also performed new type of assays called Genomic HT-SELEX (GHT-SELEX), that uses enzymatically fragmented human genomic DNA instead of random DNA sequence. Method has similarities with both Affinity-seq method by Walker et al. 2015 (PMID: 26351520) and earlier genomic SELEX methods such as methods by Singer et al. 1997 and Zimmermann et al. 2010 (PMID: 9016629 and PMID: 20541015m, respectively). Information about the experiments is encoded both in metadata and in the originally submitted sample file names. In the sample names the information is separate by underscore ""_"" symbols. For example sample named "ZNF121_TT40NTACAGC_Lysate_BatchAATA_Cycle3_R1.fastq.gz"" Means that it is derived for experiment using: 1) Protein ZNF121, 2) selection ligand where the randomised region is flanked by TT and TACAGC residues , 3) It is from experimental batch ""AATA"", and 4) From the first selection cycle of the assay. GHT-SELEX samples do not contain flanking barcodes. They were instead multiplexed with Illumina's i7 and i5 barcode system during final library preparation and have been derived from same fragmented pool.