Enhancing the DNA yield intended for microbial sequencing from a low-biomass chlorinated drinking water

This research aimed to optimize DNA yield from low-biomass (chlorinated) reverse osmosis-produced tap water by evaluating the impact of different factors during the DNA extraction procedure. The factors examined are 1) the impact of membrane materials and their pore sizes; 2) the impact of different cell densities; and 3) an alternative method for enhancing DNA yield via incubation (no nutrient spiking). To elucidate if increasing cell density could be a feasible alternative to improve DNA yield before performing downstream molecular analysis of low-biomass RO tap water, bacterial community profile from a controlled stagnation experiment was examined.