Effect of RON on GCL cell count in vehicle or CS-injected eyes.

Cell count in the GCL/100 µm was evaluated by H&E staining and Thy-1, NeuN and Brn3a immunostaining after 10 weeks of treatment with vehicle or CS in eyes submitted to sham operation or RON at 3 (upper panel) or 6 (lower panel) weeks of treatment. In sham operated eyes, CS induced a significant decrease in the GCL cell and in Thy-1, NeuN and Brn3a positive ganglion cell number, whereas in hypertensive eyes submitted to RON at 3 or 6 weeks, a significant preservation of GCL cells was observed. Data are the mean ± SEM (n = 5 retinas per group);*p**pa: pb: p