Modeling the B-cell receptor signaling on single cell level reveals a stable network circuit topology between non-malignant B cells and chronic lymphocytic leukemia cells and between untreated cells and cells treated with kinase inhibitors

This experiment had the purpose to quantify the phosphorylation of key components of the BCR pathway in CLL and healthy B cells. Thereby we assessed the signaling quantity, dynamics and interrelation of five BCR pathway proteins over a timecourse after stimulation.
Conclusion:
The BCR signaling pathway was qualitatively stable between healthy B cells and CLL cells, even under treatment with BCR signaling inhibitors. Each fluorophore was measured in a single stain with beads to allow compensation. Compensation was performed for every individual staining.