RNA- and ATAC-seq data from hESCs differentiated with Wnt and Activin with or without CPI-203

We report genome-wide changes in chromatin state and transcriptional output following Wnt-priming and subsequent Activin induced differentiation of human embryonic stem cell and the effects of BET bromodomain inhibition during Wnt-priming. The two signals, Wnt and Activin, although provided sequentially, result in stable mesendoderm differentiation (Yoney et al., 2018). We performed RNA-seq and ATAC-seq of human embryonic stem cells differentiated with Wnt and Activin and treated with BET inhibitor, CPI-203 (Selleckchem, Cat. No. S7304). Two replicates per condition for both RNA- and ATAC-seq were performed and samples were collected after 24 or 48 h.