ChEC-Seq: a robust method to identify protein-DNA interactions genome-wide

Here we show that the ChEC-Seq technique is able to differentiate the binding specificities of Esa1 and Gcn5 two chromatin-binding factors displaying widespread genome-wide associations. We also show that the ChEC-Seq technique reveals strong binding of the transcription factor Sfp1 at Ribi gene promoters. Furthermore, our data provide the first evidence that a specific DNA motif previously identified by ChEC-Seq (Albert 2019 PMID: 30804227) is in fact an in vivo binding site for Sfp1. Overall design: ChEC-Seq of Esa1 and Gcn5 in S.cerevisiae cells. ChEC-Seq of Sfp1 in wild type and pYPL245-mutant S.cerevisiae cells.