Transcription profiling of mouse AKR/J x FVB/NJ F1 versus DBA/2J x FVB F1 blood

F1 hybrids from (AKR/J x FVB/NJ) and (DBA/2J x FVB/NJ) outcrosses display a 20-fold difference in mammary tumor metastatic capacity, due to differences in inherited polymorphisms. Expression studies were performed to determine whether polymorphism-driven gene expression signatures predictive of outcome could be generated from normal tissues Experiment Overall Design: Whole blood from adult F1 animals from (AKR/J x FVB/NJ) and (DBA/2J x FVB/NJ) outcrosses was collected and arrayed on Affymetrics chip to identify basal differences in gene expression between the different genotypes源自(AKR/J × FVB/NJ)与(DBA/2J × FVB/NJ)远交组合的F1杂交系，其乳腺肿瘤转移能力存在20倍差异，该差异源于遗传多态性的不同。本研究开展表达谱分析，旨在探究能否从正常组织中获取由多态性驱动、可预测肿瘤结局的基因表达特征谱。实验总体设计：收集源自(AKR/J × FVB/NJ)与(DBA/2J × FVB/NJ)远交组合的成年F1个体的全血样本，将样本在Affymetrix基因芯片（Affymetrix）上进行杂交检测，以鉴定不同基因型间基因表达的基线差异。