Establishing a common marmoset induced pluripotent stem cell line NM-iPS by an all-in-one episomal vector approach
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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资源简介：
Epstein-Barr virus (EBV)-based episomal vector system enables persistent transgene expression, which is advantageous for efficient derivation of transgene-free induced pluripotent stem cells (iPSCs) without viral transduction. Here, we report establishment of an iPSC line from somatic fibroblasts of a neonatal common marmoset monkey (marmoset; Callithrix jacchus) using an all-in-one episomal vector that we newly developed. The established iPSC line, named NM-iPS, showed standard characteristics of pluripotency such as pluripotency-related marker expression, three germ layer differentiation, and normal karyotype (2n = 46). The novel iPSC line would be a useful resource for stem cell research using non-human primates.
提供机构：
Keio University School of Medicine
创建时间：
2022-02-20
