Circulating microRNA expression patterns and target prediction in sepsis development

To screen the circulating miRNAs which can be used to evaluate the diagnosis and prognosis of sepsis by gene microarray,and to predict its target gene and mechanism by bioinformatics analysis in sepsis. To screen the circulating miRNAs which can be used to evaluate the diagnosis and prognosis of sepsis by gene microarray,and to predict its target gene and mechanism by bioinformatics analysis in sepsis. In this study, global microRNA (miRNA) expression profiling was performed for 3 sepsis patents, 3 septic shock patients and 3 normal controls’ plasma samples by microarray. MiRNA-targets were identified by integrating the miRNA expression profiles with mRNA expression profiles and bioinformatics analysis of the matched samples. At last, we used the miRanda and Targetscan target genes databases to predict target genes of miRNAs, also analyzed the pathway by the KEGG database.