Effects of coenzyme supply methods on AcCR-mediated asymmetric reduction of prochiral ketones.
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The reactions were performed in phosphate-citrate buffer (50 mM, pH 5.0 or 7.5) containing substrates (5 mM 4′-chloroacetophenone, 10 mM 2-octanone or 15 mM ethyl acetoacetate) and different cofactor supply systems at 25°C.G-6-P: Glucose-6- phosphate.GPDH: Glucose-6- phosphate dehydrogenase.
创建时间：
2015-12-02
