Single-cell RNA sequencing of niche cells from WT, BMT and aGVHD mice

We performed scRNA-seq of non-hematopoietic cells from non-treated control (WT), BMT and aGVHD mice. Unsupervised clustering, sub-clustering based on gene expression and trajectory analysis converged on the conclusion that aGVHD likely disrupts MSC differentiation potential, especially osteogenesis. Examination of gene expression profiles in WT, BMT and aGVHD sample