microRNA (miRNA) expression profiles of murine polyploid trophoblast giant cells (TGCs) from day 9.5 implantation sites

We employed miRNA-seq to profile all miRNAs from a pure population of hand-dissected polyploid TGCs from embryonic day 9.5. These data set of polyploid-specific TGCs microRNAs will provide insights into TGCs differentiation and endoreplication. TGCs were micro-dissected from day E9.5 nine implantation sites from C57BL/J6 mice. The portion of the TGCs in direct contact with the spongiotrophoblast layer and the labyrinth layer were manually removed to avoid collecting any polyploid cells from the former or multi-nucleated syncytiotrophoblast cells from the latter.本研究采用小RNA测序（miRNA-seq）技术，对胚胎第9.5天（E9.5）手工解剖分离得到的纯化多倍体滋养层巨细胞（trophoblast giant cells, TGCs）群体中的全部miRNA进行了表达谱分析。本数据集所包含的多倍体特异性TGC的miRNA数据，将为阐明TGC的分化与核内复制机制提供重要参考。研究人员从C57BL/J6小鼠的9个胚胎着床位点中，显微解剖获取了E9.5时期的TGCs。实验过程中，研究人员手动移除了与海绵滋养层（spongiotrophoblast layer）及迷路层（labyrinth layer）直接接触的TGC区域，以避免收集到前者来源的多倍体细胞，或后者来源的多核合体滋养层细胞。