Antigen exposure reshapes chromatin architecture in central memory CD8+ T cells and imprints enhanced recall capacity [ATAC-seq]

Compared with naïve CD8+ T cells, antigen-experienced effector and central memory CD8 T cells show extensive CTCF redistribution and 3D genome reorgainzation, which underlie the transcriptomic diversification as well as recall capacity in response to secondary challenges. CreER+ WT or CTCF-floxed P14 CD8+ T cells were first transferred into B6.SJL mice followed by LCMV-Arm infection. After ≥30 days, the immune mice were treated with tamoxifen for two days, and CD62L+ P14+ central memory (Tcm) cells were sorted for ATAC-seq analysis