CIL:40241, Rattus norvegicus, CNS neuron (sensu Vertebrata), hippocampal neuron. In Cell Image Library
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Rat brain was perfused with 4% paraformaldehyde and sectionedrain section was immunolabeled for PMCA1a using CR1a antibody from Emanuel Strehler (1:2000). Secondary antibody was conjugated to Alexa Fluor 568. Section was then counterstained with Hoescht 33342 (Invitrogen) to visualized all cell nuclei. The sections were coverslipped in Gelvatol. Images were acquired using a BioRad RTS2000 with a Nikon 60X N.A. 1.4 oil-immersion objective. 46 x 19 maximum projection images were tiled together to generate this composite image.
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UC San Diego Library Digital Collections
创建时间：
2021-06-17
