c-Myc supports polyploidy and prevents senescence in the murine placenta [RNA-seq]

To understand differential gene expression between wildtype and c-Myc null mouse trophoblast giant cells. TGCs from 9.5 dpc stage were dissected as mentioned previously (Hannibal and Baker, 2016) cold PBS and embryos were used for genotyping. Total RNA from TGCs was isolated using TRIzol (#15596026, Thermo Fisher Scientific). rRNA was depleted using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB # E6310L). cDNA synthesis was performed using NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7760S) and NEBNext Multiplex Oligos for Illumina (Index Primer Set 1) (NEB # E7335S). Sequencing was performed using an Illumina NextSeq 500 with 75bp single read runs.