Composition of the standard and selective media (based on one litre of medium) used to isolate soil bacteria.

a Oil–Diesel engine oil (Rotella diesel engine oil, Shell, Montreal, QC). b Crude Oil was Coated onto the medium after solidification (from Gulf of Mexico, provided by Montreal pipeline). c Crude Oil was mixed with the growth medium (Montreal pipeline). d MgSO4 (739 mg / L), KNO3 (76 mg / L), KCl (65 mg / L), KH2PO4 (4.1 mg / L). e MnSO4*7H2O (6 mg / L), ZnSO4*7H2O (2.65 mg / L), H3BO3 (1.5 mg / L), CuSO4 (0.13 mg / L), Na2MoO4*2H2O (0.002 mg / L), KI (0.75 mg / L). Each medium contained 100 mg / L of cycloheximide.