Apple RNA-seq

The five accessions included three cultivated varieties (Weiqinni, Calville Rouge and Riskin) and two wild varieties (M. sikkimensis and M. coronaria). Samples were collected at various stages of fruit development, including fruitlet (S1), expanding (S2) and ripening (S3). Fruits were considered mature when they no longer showed an increase in size, exhibited fruit blush along with the disappearance of background color, and had a change in seed color from pale green to brown; these observations were combined with existing records of fruit maturity dates.